全文获取类型
收费全文 | 1434篇 |
免费 | 78篇 |
国内免费 | 9篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 31篇 |
妇产科学 | 204篇 |
基础医学 | 164篇 |
口腔科学 | 33篇 |
临床医学 | 218篇 |
内科学 | 180篇 |
皮肤病学 | 11篇 |
神经病学 | 38篇 |
特种医学 | 108篇 |
外科学 | 208篇 |
综合类 | 39篇 |
一般理论 | 1篇 |
预防医学 | 106篇 |
眼科学 | 1篇 |
药学 | 55篇 |
中国医学 | 2篇 |
肿瘤学 | 120篇 |
出版年
2019年 | 13篇 |
2018年 | 16篇 |
2015年 | 15篇 |
2014年 | 14篇 |
2013年 | 28篇 |
2012年 | 40篇 |
2011年 | 43篇 |
2010年 | 23篇 |
2009年 | 30篇 |
2008年 | 38篇 |
2007年 | 53篇 |
2006年 | 60篇 |
2005年 | 38篇 |
2004年 | 41篇 |
2003年 | 62篇 |
2002年 | 38篇 |
2001年 | 46篇 |
2000年 | 55篇 |
1999年 | 38篇 |
1998年 | 27篇 |
1997年 | 20篇 |
1996年 | 27篇 |
1995年 | 21篇 |
1994年 | 34篇 |
1993年 | 28篇 |
1992年 | 40篇 |
1991年 | 49篇 |
1990年 | 42篇 |
1989年 | 37篇 |
1988年 | 37篇 |
1987年 | 42篇 |
1986年 | 35篇 |
1985年 | 28篇 |
1984年 | 18篇 |
1983年 | 23篇 |
1982年 | 11篇 |
1981年 | 20篇 |
1980年 | 13篇 |
1979年 | 16篇 |
1978年 | 18篇 |
1977年 | 24篇 |
1976年 | 23篇 |
1975年 | 25篇 |
1974年 | 28篇 |
1973年 | 20篇 |
1972年 | 12篇 |
1971年 | 11篇 |
1970年 | 15篇 |
1969年 | 12篇 |
1968年 | 14篇 |
排序方式: 共有1521条查询结果,搜索用时 31 毫秒
101.
J E Byles R W Sanson-Fisher S Redman A L Reid M Agrez 《Cancer Detection and Prevention》1992,16(4):245-252
This study examines the prevalence of colorectal cancer screening among individuals in the general community. The survey was undertaken as part of a large scale general population survey of health practices and attitudes. A sample of 1090 people aged 40 years and over with no previous history of colorectal cancer or other predisposing condition was interviewed. Only 56% of this group reported that they regularly checked their bowel movements, the toilet bowl, or the toilet paper for signs of rectal bleeding. Only 13% could recall a doctor ever advising them to check for rectal bleeding; and 21% could recall a doctor asking them if they had ever noticed blood in their bowel movements. More involved practitioner-based procedures, such as digital rectal examination, fecal occult blood testing, endoscopy, and barium enema, were reported by only a minority of participants. Analysis of screening rates in the 9.3% of people who reported a family history of colorectal cancer revealed that this higher risk group was no more likely to be screened than those at average risk. 相似文献
102.
103.
Expression and subcellular localization of cyclin D1 protein in epithelial ovarian tumour cells 总被引:1,自引:0,他引:1
Dhar KK Branigan K Parkes J Howells RE Hand P Musgrove C Strange RC Fryer AA Redman CW Hoban PR 《British journal of cancer》1999,81(7):1174-1181
The expression of cyclin D1 protein in tumour sections from 81 patients with epithelial ovarian cancer was analysed using immunohistochemistry. The tumours that overexpressed cyclin D1 in more than 10% of neoplastic cells were considered positive. Thus overexpression of cyclin D1 was observed in 72/81 (89%) of the cases examined. Protein was detected in both the nucleus and the cytoplasm in 24/81 (30%) and localized exclusively in the cytoplasm in 48/81 (59%) of the tumours. Cyclin D1 was overexpressed in both borderline and invasive tumours. There was no association between protein overexpression and tumour stage and differentiation. Furthermore, no correlation between cyclin D1 expression and clinical outcome was observed. However, in tumours overexpressing cyclin D1 (n = 72), the proportion displaying exclusively cytoplasmic localization of protein was higher in those with serous compared with non-serous histology (P = 0.004, odds ratio 4.8, 95% confidence interval 1.4-19.1). Western analysis using a monoclonal antibody to cyclin D1 identified a 36 kDa protein in homogenates from seven tumours displaying cytoplasmic only and one tumour demonstrating both nuclear and cytoplasmic immunostaining. Using restriction fragment length polymorphism polymerase chain reaction and PCR-multiplex analysis, amplification of the cyclin D1 gene (CCND1 was detected in 1/29 of the tumours demonstrating overexpression of cyclin D1 protein. We conclude that deregulation of CCND1 expression leading to both cytoplasmic and nuclear protein localization is a frequent event in ovarian cancer and occurs mainly in the absence of gene amplification. 相似文献
104.
Phase II trial of oral estramustine, oral etoposide, and intravenous paclitaxel in hormone-refractory prostate cancer. 总被引:2,自引:0,他引:2
D C Smith P Esper M Strawderman B Redman K J Pienta 《Journal of clinical oncology》1999,17(6):1664-1671
PURPOSE: To evaluate the combination of intravenous (IV) paclitaxel, oral estramustine, and oral etoposide in patients with advanced hormone-refractory prostate cancer. PATIENTS AND METHODS: Forty patients with carcinoma of the prostate that was progressing despite hormonal therapy and who had undergone antiandrogen withdrawal (if previously treated with an antiandrogen) were enrolled onto this phase II trial. Patients were treated with oral estramustine 280 mg tid and oral etoposide 100 mg/d for 7 days, with paclitaxel 135 mg/m(2) IV over 1 hour on day 2 of each 21-day treatment cycle. Patients received a maximum of six cycles of therapy. RESULTS: Thirty-seven patients were assessable for response. Twenty-two had measurable disease at baseline; response was not assessable in six of these patients. Overall response was 45% (10 of 22 patients; 95% confidence interval [CI], 24% to 68%), and response was 63% (10 of 16) in assessable patients. Twenty-six patients had a > or = 50% decrease from their baseline prostate-specific antigen levels during therapy, for a response rate of 65% (95% CI, 48% to 79%) by this criterion. Median duration of response was 3.2 months, with an estimated median survival of 12.8 months. Major toxicities of therapy were leukopenia (eight patients had > or = grade 4 leukopenia) and anemia. Hematologic toxicity seemed to be associated with liver metastases. Serial measurements in 24 patients using the Functional Assessment of Cancer Therapy-Prostate (FACT-P) showed no significant change in quality of life (QOL) as a result of therapy. CONCLUSION: The combination of IV paclitaxel, oral estramustine, and oral etoposide is active in patients with advanced prostate cancer. The regimen is tolerable and does not have a significant impact on QOL as measured by the FACT-P in a limited sample of patients. 相似文献
105.
S Redman 《Physiological reviews》1990,70(1):165-198
106.
L. Provini S. Redman Dr. P. Strata 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1968,6(3):216-233
Summary Stimulation of forelimb and hindlimb areas of the sensorimotor cortex in the cat evokes in the lobus anterior of the cerebellum an early response at latency of 3–3.5 msec due to the mossy fibre input (MF) and a later response at latency of 13–16 msec due to the climbing fibre (CF) input.In the pars intermedia these two types of responses are organized in a somatotopic manner: the hindlimb area projects in lobuli HIV and HIII whereas the forelimb area projects to lobulus HV. In the vermis a somatotopic arrangement is less clear. Both forelimb and hindlimb areas of the sensorimotor cortex project to lobuli III, IV and V: on a maintained somatotopy in a caudo-rostral direction there is a tendency for the hindlimb area of the sensorimotor cortex to be well represented in a longitudinal strip close to the paravermal sulcus. This arrangement in the vermis is evident for the CF pathways, but more difficult to demonstrate for the MF pathways.The forelimb area of the sensorimotor cortex projects to those areas of the lobus anterior impinged upon by the forelimb nerves through both the MF and CF systems and the same holds true for the hindlimb area and the hindlimb nerves. 相似文献
107.
Weimin Sun Ben Anderson Joy Redman Aubrey Milunsky Arlene Buller Matthew J McGinniss Franklin Quan Arturo Anguiano Stephen Huang Feras Hantash Charles Strom 《Genetics in medicine》2006,8(6):339-345
PURPOSE: The study's purpose was to understand the molecular basis for different clinical phenotypes of the 5T variant, a tract of 5 thymidines in intron 8 of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, which disrupts processing of CFTR mRNA and reduces synthesis from the corresponding CFTR alleles. METHOD: We analyzed the polymorphic TG dinucleotide repeat adjacent to the 5T variant in intron 8 and the codon 470 in exon 10. Patients selected for this study were positive for both the 5T variant and the major cystic fibrosis mutation, Delta F508. Almost all Delta F508 mutation alleles occur in a 10TG-9T-470M haplotype. Therefore, it is possible to determine the haplotype of the 5T variant in trans. RESULTS: Of the 74 samples analyzed, 41 (55%) were 11TG-5T-470M, 31 (42%) were 12TG-5T-470V, and 2 (3%) were 13TG-5T-470M. Of the 49 cases for which we had clinical information, 17.6% of females (6/34) and 66.7% of males (10/15) showed symptoms resembling atypical cystic fibrosis. The haplotype with the highest penetrance in females (42% or 5/12) and more than 80% (5/6) in males is 12TG-5T-470V. We also evaluated 12 males affected with congenital bilateral absence of vas deferens and positive for the 5T variant; 10 of 12 had the 12TG-5T-470V haplotype. CONCLUSION: Overall, the 5T variant has a milder clinical consequence than previously estimated in females. The clinical presentations of the 5T variant are associated with the 5T-12TG-470M haplotype. 相似文献
108.
M S Dryden M Wilkinson M Redman M R Millar 《The British journal of general practice》1994,44(380):114-117
BACKGROUND. Chlamydia trachomatis is frequently overlooked as a cause of dysuria and urinary frequency in general practice patients. AIM. This study set out to determine the impact of performing chlamydial antigen detection on sterile pyuria samples from patients aged 16-65 years and which were submitted to a hospital microbiology laboratory by general practitioners in the Winchester health district for routine microbiological investigations. METHOD. Chlamydial antigen detection was performed by enzyme immunoassay and direct immunofluorescence. The cost of performing the test was estimated. In the first year of the study (1991) questionnaires were sent to general practitioners whose patients had a positive test result. RESULTS. A total of 1025 samples of sterile pyuria were received at the laboratory between January 1991 and March 1993. Chlamydial antigen was detected in 54 samples (5%); 22 men and 32 women aged between 16 and 57 years (mean 25 years). The detection rate was highest in the 16-20 years age group (22% of men had a positive sample and 7% of women). Completed questionnaires from 27 general practitioners revealed that 59% of their patients were referred to the genitourinary clinic for treatment and contact tracing. The others were treated by the general practitioner. The cost of the screening programme per cure in this population was estimated to be 246 pounds. CONCLUSION. C trachomatis is a significant pathogen which may go unrecognized and untreated. The cost, medically and financially, of screening for this pathogen and treating infected patients and contacts is likely to be less than ignoring it, particularly if screening is confined to the 16-30 years age group. General practitioners should consider the diagnosis of chlamydial infection in young adult patients with sterile pyuria, and microbiology laboratories should screen sterile pyuria samples for chlamydial antigen. 相似文献
109.
Liu JQ; Bai XF; Shi FD; Xiao BG; Li HL; Levi M; Mustafa M; Wahren B; Link H 《International immunology》1998,10(8):1139-1148
Induction of mucosal tolerance by inhalation of soluble peptides with
defined T cell epitopes is receiving much attention as a means of
specifically down-regulating pathogenic T cell reactivities in autoimmune
and allergic disorders. Experimental autoimmune encephalomyelitis (EAE)
induced in the Lewis rat by immunization with myelin basic protein (MBP)
and Freund's adjuvant (CFA) is mediated by CD4+ T cells specific for the
MBP amino acid sequences 68-86 and 87-99. To further define the principles
of nasal tolerance induction, we generated three different MBP peptides
(MBP 68-86, 87-99 and the non- encephalitogenic peptide 110-128), and
evaluated whether their nasal administration on day -11, -10, -9, -8 and -7
prior to immunization with guinea pig MBP (gp-MBP) + CFA confers protection
to Lewis rat EAE. Protection was achieved with the encephalitogenic
peptides MBP 68-86 and 87-99, MBP 68-86 being more potent, but not with MBP
110-128. Neither MBP 68-86 nor 87-99 at doses used conferred complete
protection to gp-MBP-induced EAE. In contrast, nasal administration of a
mixture of MBP 68-86 and 87-99 completely blocked gp-MBP-induced EAE even
at lower dosage compared to that being used for individual peptides. Rats
tolerized with MBP 68-86 + 87-99 nasally showed decreased T cell responses
to MBP reflected by lymphocyte proliferation and IFN-gamma ELISPOT assays.
Rats tolerized with MBP 68-86 + 87-99 also had abrogated MBP-reactive
IFN-gamma and tumor necrosis factor-alpha mRNA expression in lymph node
cells compared to rats receiving MBP 110-128 nasally, while similar low
levels of MBP-reactive transforming growth factor-beta and IL-4 mRNA
expressing cells were observed in the two groups. Nasal administration of
MBP 68-86 + 87-99 only slightly inhibited guinea pig spinal cord
homogenate-induced EAE, and passive transfer of spleen mononuclear cells
from MBP 68-86 + 87-99-tolerized rats did not protect naive rats from EAE.
Finally, we show that nasal administration of MBP 68-86 + 87-99 can reverse
ongoing EAE induced with gp-MBP, although higher doses are required
compared to the dosage needed for prevention. In conclusion, nasal
administration of encephalitogenic MBP peptides can induce antigen-specific
T cell tolerance and confer incomplete protection to gp-MBP-induced EAE,
and MBP 68-86 and 87-99 have synergistic effects. Non-regulatory mechanisms
are proposed to be responsible for tolerance development after nasal
peptide administration.
相似文献
110.
This report describes the discovery and characterization of the novel Cw*0346 allele. 相似文献